EnSightTM

The EnSightTM Multimode Plate Reader for 96- and 384-well microplates from PerkinElmer allows us to measure a wide range of detection technologies like absorption, fluorescence intensity, time-resolved fluorescence and ultra-sensitive luminescence in a fast and more time-efficient manner. The Plate Reader also comes with the Kaleido™ Data Acquisition and Analysis Software enabling us to achieve a more productive workflow.

 

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Büchi Miniclave

Some time ago, our new lab-equipment arrived! It's a pressure autoclave from Büchi ("Miniclave steel type 3") which gives us the opportunity to heat up reactions to 200 °C under high pressure. We will use this device for high pressure (more than 40 bars) hydrogenation in our organo-synthetic lab. It is good to have this support in the laboratory!

 

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Opentrons OT-2 Pipetting Robot

For our SupraSmartLab we got a new pipetting robot  from Opentrons. With the help of this device it is possible to automate many of our protocols and to therefore fasten up our spectroscopy projects! Due to the custom made cuvette holders and the anyway available compatibility with currently used tips and labware we can transfer all our pipetting processes on our new roboter!

 

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CD/FDCD Spectrometer

For our circular dichroism studies we are using a Jasco J1500 CD spectrometer. 

In the current setup we are not only able to detect CD spectra for circular dichroistic behaviour of chiral samples with a transmitting light beam, but also FDCD spectra. 

FDCD is the abbreviation for fluorescence-detected CD spectroscopy which measures the differential fluorescence intensity that results from excitation with left-handed and right-handed circularly polarized light. While a CD spectrum can be understood as analogues technique to an absorption spectrum, FDCD can be understood as an excitation spectrum. 

Despite FDCD spectroscopy requires luminescent materials to be probed for their chiroptical properties, it offers a higher sensitivity then "normal" CD-spectroscopy.  

 

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Fluorescence spectrometer with Automatic Titration Unit

For our photophysical investigations we are not only unsing the fluorescence spectrometers provided by the INT (Horiba FL3 and Cary Eclipse), but also use the FP8300 by JASCO. 

For higher reproducibility in our fluorescence based supramolecuar binding studies we are taking advantage of a automatic titration unit (top in the picture).

 

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Isothermal Titration Calorimeter

Our MircoCal PEAQ-ITC by Malvern Panalytical allows us to measure all binding parameters in a single experiment. It is a highly sensitive isothermal titration colorimeter which can analyze samples as little as 10 µg.

 

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Combiflash

Our CombiFlash® Rf 150 by TELEDYNE ISCO helps us to improve and fasten up column chromatography to a maximum. With the different column sizes from 4 grams to 330 grams, the possibility to use Silica, Aluminiumoxide or modified Silica as well as the different column loading techniques we an purify almost every crude. Besides the well know column separation also TLC checks can be fastened up by quickly running a short column and checking the UV spectrum. Due to the applied internet connection it is also possible to check the column progress from the office computer or any other place.

 

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UV-Vis Spectrometer

For our photophysical investigations we are not only unsing the UV-Vis spectrometer provided by the INT, but also use the V-730 by JASCO. We use it for basic absorbance spectra measurements and also for kinetic studies.

 

 

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Plate Reader

We are happy to welcome our new colleague in our Lab - a PlateReader! With the help of our JASCO PlateReader we can measure many samples in a short time, which allows us to get a lot more results and to do many reproducibility tests. In combination with our Robot we save a lot of time and money and are on a good way to our targeted "SupraSmartLab". 

 

 

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Luzchem Photoreactor

For photolytical reactions a photoreactor from Luzchem, Typ LZC-4X, can be used. The lightsources are UV-B-lamps with ?Max = 254 nm and ?Max = 365 nm. By use of the lightsource with 254nm the reaction chamber has to be flooded with nitrogen at least 30 minutes to devoid the buildung of ozone. A ventilation system attached to the exhaust system ensures optimum safety during reactions.

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